A candidate reference method for determination of bilirubin in serum. Test for transferability.

نویسندگان

  • B W Perry
  • B T Doumas
  • D D Bayse
  • T Butler
  • A Cohen
  • W Fellows
  • C C Garber
  • B Howell
  • T Koch
  • S Krishnamurthy
  • A Louderback
  • R B McComb
  • D Miller
  • R R Miller
  • R N Rand
  • R Schaffer
چکیده

Each of 10 laboratories, using portions of a single crystalline bilirubin preparation, prepared bilirubin standards in solutions of bovine serum albumin. The standards were prepared on two days. The standards and two control sera were analyzed by a modified Jertdrassik-Grof method, in duplicate, on the same day the standards were prepared. The mean molar absorptivity of the alkaline azobilirubin at 598 nm was 75 080 L mol1cm1 with a standard deviation of 760. Mean values for the two controls were 41.4 and 113.5 mg/L, with standard deviations of 0.9 and 1.4 mg/L, respectively.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

The transferability of a candidate reference method for determination of creatinine in serum.

We developed a candidate reference method for the determination of creatinine in serum. For the acceptance of a reference method it is important that it be rigorously validated against a definitive method and that the method can be transferred from one laboratory to another. This study focussed on the transferability and consisted of two parts: introduction and familiarization with the method i...

متن کامل

Candidate reference method for determination of total bilirubin in serum: development and validation.

This candidate Reference Method for measuring total bilirubin in serum is based on the Jendrassik-Gróf principle (Clin Chem 29: 297-301, 1983). Standard Reference Material no. 916 bilirubin (National Bureau of Standards) is used as the standard. Bilirubin standard solutions may be prepared either in human serum or in 40 g/L albumin solution (human or bovine), because we found the molar absorpti...

متن کامل

A candidate reference method for determination of total protein in serum. I. Development and validation.

We developed a candidate Reference Method for measuring total serum protein by use of the biuret reaction. The method involves a previously described biuret reagent (Clin. Chem. 21: 1159, 1975) and Standard Reference Material (SRM) 927 bovine albumin (National Bureau of Standards) as the standard. At 25 degrees C, color development for 30 or 60 min provides identical serum protein values. Gluco...

متن کامل

Determination of reference ranges using medical laboratory date

In order to have reliable reference ranges and to minimize the effects of variables such as the procedure of specimen collection, geographical location, dietary habits, life style and possible errors during the performance of tests, it  is recommended that each medical laboratory establish it's own reference values. Idealy, the reference ranges Should be determined by analysis of specimens from...

متن کامل

A Comparison between Transcutaneous Bilirubin (TcB) and Total Serum Bilirubin (TSB) Measurements in Term Neonates

Background: Transcutaneous bilirubinometry (TCB) is a simple method for estimating bilirubin levels in neonates. This method is noninvasive, quick, and painless. We aimed to compare serum and cutaneous bilirubin measurements in term neonates.Method: In this descriptive cross-sectional study, 200 neonates with icter with birth weights of at least 2500 grams were studied. TCB was measured using a...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Clinical chemistry

دوره 29 2  شماره 

صفحات  -

تاریخ انتشار 1983